Nori Sheep CD24 ELISA Kit
$461.00 – $832.00
DataSheet CoA SDS
This ELISA kit is for quantification of CD24 in ovine. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for CD24 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD24 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for CD24 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of CD24 bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for CD24: Cluster of differentiation 24, CD24A, heat stable antigen CD24 (HSA)
This product is for laboratory research use only not for diagnostic and therapeutic purposes or any other purposes.
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Description
Nori Sheep CD24 ELISA Kit Summary
Alternative names for CD24: Cluster of differentiation 24, CD24A, heat stable antigen CD24 (HSA)
Alternative name for sheep: Ovine, lamb, goat
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number |
XP_004011283 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 25 pg/mL |
| Detection Range | 125-8000 pg/mL |
| Specificity | Natural and recombinant sheep CD24 (HSA) |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Signal transducer CD24 also known as cluster of differentiation 24 or heat stable antigen CD24 (HSA) is a protein that is encoded by the CD24 gene.[1] CD24 is a cell adhesion molecule and is a sialoglycoprotein expressed at the surface of most B lymphocytes and differentiating neuroblasts. It is also expressed on neutrophils[2] and neutrophil precursors from the myelocyte stage onwards. CD24 is anchored via a glycosyl phosphatidylinositol (GPI) link to the cell surface. The protein also contributes to a wide range of downstream signaling networks and is crucial for neural development.[3] Cross-linking of CD24 on the surface of neutrophils induces apoptosis, and this appears to be defective in sepsis.[4] CD24 is a signal transducer modulating B cell activation responses and is a very short peptide with a glycosyl phosphatidylinositol membrane anchor.[5] CD24 is an effector of HIF-1-driven primary tumor growth and Metastasis.[6] CD24 is a potential early tumor marker gene correlates with p53 mutation and tumor differentiation.[7] CD24, a signal-transducing molecule expressed on human B cells, is a major surface antigen on small cell lung carcinomas.[8]
References
- Hough MR, et al. (1994). Genomics. 22 (1): 154–61.
- Elghetany MT, Patel J (2002). American Journal of Hematology. 71 (4): 348–9.
- Gilliam DT, et al. (2017). Neurobiology of Disease. 99: 133–144.
- Parlato M, et al. (2014). Journal of Immunology. 192 (5): 2449–59.
- Kay R, et al. (1991) J Immunol 147 (4), 1412-1416.
- Thomas S, et al. (2012) Cancer Res 72 (21), 5600-5612.
- Huang LR and Hsu HC. (1995) Cancer Res 55 (20), 4717-4721.
- Jackson D, et al. (1992) Cancer Res 52 (19), 5264-5270.
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