Nori Human IL-18 BPa ELISA Kit
$461.00 – $832.00
DataSheet CoA SDS
This ELISA kit is for quantification of IL-18 BPa in human. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for IL-18 BPa has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-18 BPa present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for IL-18 BPa is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of IL-18 BPa bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for asprosin: Fibrillin, fibrillin-1
This product is for laboratory research use only not for diagnostic and therapeutic purposes or any other purposes.
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Description
Nori Human IL-18 BPa ELISA Kit Summary
Alternative names for IL-18 BPa: Interleukin 18 binding protein, IL18BP
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | O95998 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 30 pg/mL |
| Detection Range | 0.156-10 ng/mL |
| Specificity | Natural and recombinant human IL-18 BPa |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Interleukin-18-binding protein is a protein that in humans is encoded by the IL18BP gene.[1][2]
The protein encoded by this gene is an inhibitor of the proinflammatory cytokine IL18. This protein binds to IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production. This protein is constitutively expressed and secreted in mononuclear cells. The expression of this protein can be enhanced by IFN-gamma. An elevated level of this protein is detected in the intestinal tissues of patients with Crohn’s disease. Three transcript variants encoding the same protein have been found for this gene.[2] A large proportion blood basophils from patients with asthma express IL-18 and IL-18BP.[3] Mast cells and basophils are implicated in the pathogenesis of asthma via an IL-18-related
mechanism. IL-18, IL-18R and IL-18BP expression in eosinophil are involved in the inflammatory reaction of asthma. Asthma is very likely to be determined by balance of IL-18/IL-18BP/IL-18R expression in inflammatory cells.[4] IL-18BP messenger transcript and protein are significantly
increased in surgically resected specimens from active Crohn’s disease compared with control patients, correlating with an up-regulation of IL-18.[5] Interleukin-18 binding protein production is regulated in blood and synovial cells from patients with rheumatoid arthritis.[6]
References
- Aizawa Y, et al. (1999) FEBS Lett. 445 (2-3), 338-342.
- Novick D, et al. (1999). Immunity. 10 (1): 127–36.
- Wang Z, et al. (2018) Scand. J. Immunol. 87 (5), e12658.
- Zhang H, et al.(2018) J. Cell. Mol. Med. 22 (1), 354-373.
- Corbaz A, et al. J. (2002) (2002) Immunol. 168 (7), 3608-3616.
- Kawashima M, et al. (2004). Arthritis Rheum. 50 (6): 1800–5
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